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Chromosome evolution paper

INTERNATIONAL POULTRY GENOME MAPPING WORKSHOP - RECOMMENDATIONS

The workshop agrees on the need for a basic map of evenly spaced polymorphic markers anchored to loci of known physical location. In order to further this goal the workshop makes the following recommendations.

Reference populations and the chicken genome map

1. The workshop recognizes the primary reference populations developed by the Michigan State University-USDA, ARS Poultry Genome Mapping Project, East Lansing, USA (L. B. Crittenden) and the Compton Laboratory, Institute for Animal Health, UK (N. Bumstead) as the basic reference populations for developing the chicken molecular genetic map. It is requested that probes be exchanged between the two Laboratories, so that the two maps can be coordinated, and that a consensus map be made available when a high proportion of linkage groups are associated on the two maps.
2. The Workshop recommends that DNA samples of the reference populations (56 from the Compton Laboratory and 52 from the East Lansing Laboratory) shall be made available to Laboratories other than the East Lansing and Compton Laboratories that have indicated a commitment to make a major investment in developing the chicken gene map. These Laboratories will be referred to as "Other Laboratories". First, parental DNA shall be sent to the Other Laboratories for testing for polymorphisms. Then one or both of the backcross sets will tested for segregation. Those Other Laboratories that choose to analyse both sets will contribute to the development of the consensus map by associating linkage groups from the two maps. It is expected that five or six Other Laboratories will participate in map development, at first, and that this number will grow to no more than ten or twelve.
3. The Workshop recommends that the microcomputer program, GENE LINK (or other program agreed upon after review of GENE LINK), shall be distributed with the DNA panel from East Lansing and Compton Laboratories with the current map entered so that new markers can be added to the map immediately. Current individual genotypes and segregation data will be contained in the GENE LINK disk distributed. Raw data shall be returned to the Compton and East Lansing Laboratories for final map development, approval by the ISAG Committee on Poultry Genome Mapping and distribution and/or publication. Efforts will be made to place enough markers on both maps to develop a consensus map. The Workshop-sponsored map will be updated annually, and perhaps published as a supplement to Animal Genetics if such a supplement is approved by the ISAG at it's 1994 Conference. This will be a publication of the Workshop and those who did the most work on the publication will be given credit for compilation. Other Laboratories that place markers on the map are free to use the consensus map to orient those markers in their individual publications but need not include members of the East Lansing or Compton Laboratories as authors unless they have made specific new contributions to the work.
4. The Workshop recommends that all probes that represent loci on the Workshop-sponsored map shall be freely exchanged among the Workshop collaborators prior to formal individual publication and become publicly available upon such individual publication. Probe exchange among Workshop collaborators should take place within six months of placement on the map, and early publication is encouraged.
5. The Workshop recommends that laboratories that intend to develop only an occasional marker shall communicate with the East Lansing or Compton Laboratories who may either map them or recommend another Laboratory that has received mapping panel DNA.

Other issues

6. The Workshop appointed D. W. Burt, N. Bumstead, and L. B. Crittenden to establish a database of mapping and related data using G-Base in conjunction with PigMap in Edinburgh. Initially remote input shall be established for the East Lansing and Compton laboratories. Input can later be extended to other laboratories that may coordinate the input of physical and classical mapping data. This database will be available by remote access to laboratories contributing to map development. If funds become available, a duplicate database will be established at a coordinating laboratory in North America and data will be transmitted to the National Agricultural Library, Beltsville, MD, USA to help establish a public access database for several species of animals. This would be in addition to and/or coordinated with publication in the Somes reports and O'Brien Genetic Maps currently updated by J. Bitgood, University of Wisconsin. Electronic publication may ultimately replace these hard copy publications.
7. The Workshop recommends that classical loci be named according to guidelines published by Somes. Loci identified by molecular probes will be named according to guidelines established by the human and mouse genetics communities as exemplified by their use in G-Base. The Workshop asks M. Tixier-Boichard to make these guidelines readily available and monitor their use on the Workshop-sponsored map.
8. The workshop recommends initiation of a program to coordinate and unify classical, molecular and physical maps.
9. The workshop recommends that information on resource populations for mapping of genes of biological or agricultural interest be collected and made widely available.

This page was last modifed on 25th November 1999
Converted to HTML by Andy Law from an original Draft by Lymen B. Crittenden
© Copyright Roslin Institute 1999