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Report of the 8th Workshop of the ISAG BoLA Nomenclature
Committee.
- Progress
from 1996 BoLA workshop
At
the 7th BoLA workshop, it was decided that the workshop report
would contain rules for sequence-based nomenclature of class I
and class II alleles; that the available class II alleles would
be assigned names; and that all of the information on typing and
nomenclature of BoLA genes would be made available through a world-wide-web
site. Two workshop reports were produced, and published in Animal
Genetics in July 1997. Simultaneously with the publication of
the reports, the BoLA
Nomenclature web site was established.
- Election
of committee members
Three
members of the committee, Leif Andersson, Jan van der Poel and
Noelle Cockett, indicated their wish to stand down. Nominations
were received for the remaining committee members and for two
new members, Dr Karin Saker (Virginia Maryland Regional College
of Veterinary Medicine, USA) and Dr Loren Skow, Texas A&M University,
USA), prior to the workshop. However, since Dr Saker is not currently
an ISAG member, her nomination was not valid. No further nominations
were made at the workshop and the nominees were elected by show
of hands. The current committee membership is therefore: Chris
Davies, Shirley Ellis, Evert Hensen, Harris Lewin, George Russell,
and Loren Skow. Election of chair and secretary for 1998-2000
will be done within the committee by e-mail, since only half of
the committee could attend the workshop.
- Workshop
Discussion
- Class
I typing and nomenclature
The
discussion of class I genes and locus identification emphasised
the importance of correlating the established serological data
with sequence information. To aid this process, those labs with
access to BoLA typing reagents (currently Urbana and Roslin)
have agreed to make them available to other workers by arrangement.
A proposal for class I nomenclature based on serology and sequence
will be prepared by the BoLA committee for the next workshop
- Class
II typing and nomenclature
Since
the publication of the 7th BoLA workshop report, 11 new DRB3
alleles were named, one allele has been withdrawn, and one allele
has been renamed (details below).
It was decided that in future, details of acceptance of new
BoLA sequences and nomenclature of alleles would be published
through the BoLA web site, and would not be included in workshop
reports submitted to journals.
BoLA class II summarySequence-based nomeclature was set up following
the 1996 BoLA workshop.The 1996 workshop report named:
63 DRB3 alleles of 40 major types;
39 DQA alleles of 20 major types;
37 DQB alleles of 20 major types;
The following alleles were named susequently and have been added to
the BoLA web site. DRB3 alleles- 11 new alleles, with 3 new
major types added.
|
Allele
|
Accession
number
|
Author/contributor
|
|
DRB3*0303
|
AJ
002003
|
J.C.
Maillard, unpublished
|
|
DRB3*1203
|
|
Y.
Aida, unpublished
|
|
DRB3*1302
|
|
Y.
Aida, unpublished
|
|
DRB3*1602
|
U90921
|
Mikko
et al. 1997
|
|
DRB3*1902
|
AJ
002004
|
J.C.
Maillard, unpublished
|
|
DRB3*1903
|
Z82997
|
K.T.
Ballingall, unpublished
|
|
DRB3*2707
|
AJ
002002
|
J.C.
Maillard, unpublished
|
|
DRB3*3203
|
AJ
002000
|
J.C.
Maillard, unpublished
|
|
DRB3*4101
|
U90922
|
Mikko
et al. 1997
|
|
DRB3*4201
|
AJ
001999
|
J.C.
Maillard, unpublished
|
|
DRB3*4301
|
AJ
002001
|
J.C.
Maillard, unpublished
|
DRB3 alleles removed/renamedDRB3*0102 (Fraser
et al. 1996) This allele was found to contain a single base mutation
in cloning, and was in fact identical to DRB3*0101.
Action - allele DRB3*0102 was removed as an allele name,
but reserve it as referring to the mutant allele.DRB3*3801 (K.T.
Ballingall, unpublished) Error in nomenclature - should be with DRB3*1901
major group.
Action - renamed as DRB3*1903. The name DRB3*3801
will not be used for a new allele.DQ alleles No new DQA or
DQB alleles since the published report, but three full-length
cDNAs were reported for alleles DQA*25012 (Y14020), DQA*2602
(Y14021), and DQA*27012 (Y14022) determined by Keith Ballingall
(ILRI). |